Autoantibodies in acquired hemolytic anemia with special reference to the LW system.
نویسندگان
چکیده
Most autoantibodies in patients with warm antibody autoimmune hemolytic anemia (AIHA) have specificity within the Rh system. Using rare cells such as -Dand cells, Weiner and Vos (1963) described specificity against normal cells (nI), partially deleted cells (pdl), and deleted cells (dl). Recently, autoantibodies which failed to react with Rh,, ,, cells that were of anti-U specificity have been described. It was suggested that the ‘Rh related” autoantibodies that cannot be identified as specific Rh antibodies may be anti-U. In the present study we examined eluates from the red cells of eight patients with AIHA using a panel of extremely rare cells and cross-absorption and elution techniques. We demonstrated autoantibody specificities not definable without the rare cells and, further, defined heterogeneity of the LW antigen. Autoantibodies with U specificity occurred in three eluates only. It was always present with an antibody of another specificity. Six of the eluates contamed anti-LW, two anti-nI, five anti-pdl, three anti-dI, and one anti-c. Absorption and elution studies using the rare Rhpositive LW-negative (Mrs. Bigelow) showed that anti-pdl may in fact represent anti-LW + LW1 and that Mrs. Bigelow may represent a weak variant of LW. Injection of her red cells into guinea pigs produced an anti-LW that reacted similarly to the antibody produced by injecting Rhpositive LW-positive cells. An analogy to the ABO is suggested that normal Rhpositive LW-positive cells represent LW1, Rh-negative LW-positive cells represent LW2, Mrs. Bigelow represents LW3 and Rh,,, 11 cells represent the only true LWnegative (1w).
منابع مشابه
The erythrocyte-coating substance in autoimmune hemolytic disease: its nature and significance.
W HEN THRICE-WASHED RED CELLS are agglutinated by rabbit anti-human-globulin sera (Coombs’ reagent), they are said to be “coated” with an “incomplete” (blocking) antibody.1 Such coating occurs in vivo in erythroblastosis fetalis as a result of placental transfer of maternal isoantibody, and in acquired hemolytic anemia of the immunologic variety2 (“autoimmune” hemolytic disease ) as a result of...
متن کاملRed cell autoantibody mimicking anti-C specificity: a rare manifestation
The association of systemic lupus erythematosus (SLE) with autoimmune hemolytic anemia (AIHA) is a known phenomenon. In many cases of AIHA, no autoantibody specificity is present. The patient’s serum reacts with all of the red blood cell (RBC) samples tested and the autoantibody appears to have broad specificity in the Rh blood group system. Occasionally, RBC autoantibodies demonstrate apparent...
متن کاملRed cell autoantibody mimicking anti-C specificity: a rare manifestation
The association of systemic lupus erythematosus (SLE) with autoimmune hemolytic anemia (AIHA) is a known phenomenon. In many cases of AIHA, no autoantibody specificity is present. The patient’s serum reacts with all of the red blood cell (RBC) samples tested and the autoantibody appears to have broad specificity in the Rh blood group system. Occasionally, RBC autoantibodies demonstrate apparent...
متن کاملRed cell autoantibody mimicking anti-C specificity: a rare manifestation
The association of systemic lupus erythematosus (SLE) with autoimmune hemolytic anemia (AIHA) is a known phenomenon. In many cases of AIHA, no autoantibody specificity is present. The patient’s serum reacts with all of the red blood cell (RBC) samples tested and the autoantibody appears to have broad specificity in the Rh blood group system. Occasionally, RBC autoantibodies demonstrate apparent...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Blood
دوره 42 3 شماره
صفحات -
تاریخ انتشار 1973